Simoa® immunoassay combines single molecule analysis with a digital ELISA readout. As official Quanterix partner organization, Chimera Biotec has the unparalleled expertise in ultra sensitive analytics to make the most out of Simoa technology.

We understand the high demands of our customer. As one of the few, we integrated sample cooling into our Simoa® HD-1-Analyzer to balance differences between individual samples within an analytical run, as well as between runs and ultimately over the course of long-term study support. This also ensures the stability of your temperature sensitive samples.

We offer Simoa® Quanterix immunoassay services.

Chimera Biotec offers full Simoa® testing service for all your bioanalytical needs. We gladly assist you with biomarker support based on the Simoa® biomarker kit menu, the transfer of in-house-developed assays or custom assay development service.

 


 

Simoa® Technology

 

Simoa® Assays are ideal for single- and multiplex detection. Target-specific antibodies are coupled to paramagnetic particles.

Analytes are captured in semi-homogenous solution and form an immunocomplex with detection antibodies, capable of generating fluorescent product.

At low concentrations, each bead-complex will contain 1 or 0 protein and is trapped into one of 200,000 femtoliter sized microcavities on the array.

The digital return signal (on/off) offers enhanced sensitivity over conventional Multiplex ELISA assays.

 

 

 

The single molecule array (Simoa) technology from Quanterix combines single molecule analysis with a digital ELISA readout. Analytes are captured in semi-homogenous solution by antibody coated magnetic beads. Beads are trapped in femtoliter sized microcavities and a digital signal (on/off) provides enhanced sensitivity over conventional ELISA. An official Quanterix partner organization, Chimera Biotec has the unparalleled expertise in ultra sensitive analytics to make the most out of Simoa technology. Utilizing sample cooling within the Simoa HD-1 analyzer instrument, Chimera takes the stability of your valuable sample material and fundamentals of ultra sensitive immunoassays very seriously.

 

 

Ultra sensitive in 4 steps

Step 1: Paramagnetic particles are covered with
              target-specific antibodies

Step 2: Analytes are captured by detection antibodies,
              capable of generating fluorescent product

Step 3: Beads are trapped in one of 200,000 femtoliter
              sized microcavities on the array.
              At low concentrations, each bead-complex will
              contain 1 or 0 protein.

Step 4: Digital return signal "on" or "off"

 


 

 

Simoa® Applications

Biomarker

Pharmacokinetik

Pharmakodynamic

 

 

Chimera’s Simoa® expertise

We support your study design with ultra sensitive sample testing.

 

Platform intrinsic advantages Our services at a glance
  • Digital ELISA read out
  • Bead based immune reaction
  • Fully automated platform
  • Ultra sensitive platform
  • Numerous off-the-shelf kits
  • In-house antibody pair testing
  • Assay development services
  • Biomarker assays
  • PK assays
  • Assay transfer
  • Assay validation

 

Take advantage of Chimera Biotec‘s more than 20 years of in-depth experience as a specialty CRO for immunoassays which go beyond the scope of what is usually considered feasible.

Benefit from the best in class in immunoassay sensitivity!

 


Simoa® immunoassay services:

Our kit-based biomarker portfolio for the Simoa® HD1-Analyzer. If you can not find your biomarker of interest, we develop a highly specific custom assay for you (Quanterix’ homebrew kit). Just contact our team of experts.

Target Item Species Matrix LLOQ (pg(ml) Datasheet
Alpha-Synuclein 102233 Human Serum, Plasma 4.12 Datasheet
A-beta 40 101672 Human CSF, Serum, Plasma 1.23 Datasheet
A-beta 42 101664 Human CSF, Serum, Plasma 0.137 Datasheet
BDNF 102039 Human CSF, Serum, Plasma 0.0293 Datasheet
c-MET 102073 Human Serum, Plasma 0.244 Datasheet
C-Peptide 100199 Human Serum, Plasma 0.021 Datasheet
Cathepsin S 102064 Human Serum, Plasma 1.95 Datasheet
CEA 102556 Human Serum, Plasma 2.33 Datasheet
CRP 102583 Human Serum, Plasma 0.686 Datasheet
CXCL13 (BCA-1) 102635 Human Serum, Plasma 0.070 Datasheet
Cytokine 3-Plex A 101160 Human Serum, Plasma 0.051, 0.011, 0.0073 Datasheet
Cytokine 3-Plex B 101319 Human Serum, Plasma 0.026, 0.023, 0.0068 Datasheet
Eotaxin (CCL-11) 101212 Human Serum, Plasma 0.17 Datasheet
G‐CSF 101235 Human Serum, Plasma 0.095 Datasheet
GFAP 102336 Human CSF, Serum, Plasma 0.686 Datasheet
GM‐CSF 102329 Human Serum, Plasma 0.0103 Datasheet
HE4/WFDC2 103059 Human Serum, Plasma 0.977 Datasheet
HIV p24 102215 Human Serum, Plasma 0.010 Datasheet
IFN-alpha 100860 Human Serum, Plasma 0.016 Datasheet
IFN-gamma 103337 Human Serum, Plasma 0.098 Datasheet
IL-10 101643 Human Serum, Plasma 0.021 Datasheet
IL-12p70 100988 Human Serum, Plasma 0.017 Datasheet
IL-12p40/IL-23 101871 Human Serum, Plasma 0.086 Datasheet
IL-13 102732 Human Serum, Plasma 0.005 Datasheet
IL-15 100794 Human Serum, Plasma 0.0062 Datasheet
IL‐17A 101599 Human Serum, Plasma 0.021 Datasheet
IL‐17A 101824 Mouse Serum, Plasma 0.206 Datasheet
IL-17C 102570 Human Serum, Plasma 0.206 Datasheet
IL-17F 101839 Mouse Serum, Plasma 0.412 Datasheet
IL-18 102700 Human CSF, Serum, Plasma 0.012 Datasheet
IL-1 alpha 101968 Human Serum, Plasma 0.010 Datasheet
IL-1 alpha 102504 Mouse Serum, Plasma 0.060 Datasheet
IL-1 beta 101605 Human Serum, Plasma 0.083 Datasheet
IL-1 beta 102517 Mouse Serum, Plasma 0.0309 Datasheet
IL-2 101635 Human Serum, Plasma 0.041 Datasheet
IL-22 101919 Mouse Serum, Plasma 0.206 Datasheet
IL-22 (total) 103071 Human Serum, Plasma 0.0103 Datasheet
IL-23 101847 Mouse Serum, Plasma 0.137 Datasheet
IL-28A 101419 Human Serum, Plasma 0.069 Datasheet
IL-3 102462 Human Serum, Plasma 0.686 Datasheet
IL-33 103093 Human Serum, Plasma 0.686 Datasheet
IL-36 beta 101808 Human Serum, Plasma 0.206 Datasheet
IL-4 100196 Human Serum, Plasma 0.039 Datasheet
IL-5 102860 Human Serum, Plasma 0.0165 Datasheet
IL-6 101622 Human Serum, Plasma 0.010 Datasheet
IL-6 102462 Mouse Serum, Plasma 0.120 Datasheet
IL-7 103277 Human Serum, Plasma 0.103 Datasheet
IL-8 100198 Human Serum, Plasma 0.0921 Datasheet
IP‐10 (CXCL10) 101132 Human Serum, Plasma 0.177 Datasheet
LIF 102394 Human Serum, Plasma 0.086 Datasheet
MCP-1 (CCL2) 101154 Human Serum, Plasma 0.153 Datasheet
MCP‐3 102382 Human Serum, Plasma 0.309 Datasheet
MMP-9 102491 Human CSF, Serum, Plasma 4.88 Datasheet
Neuro 4-Plex B 103345 Human CSF, Serum, Plasma 0.500, 9.38, 9.38, 0.125 Datasheet
Neuro 3-Plex A 101995 Human CSF, Plasma 0.675, 0.142, 0.063 Datasheet
Neuro 4-Plex A 102153 Human CSF, Serum, Plasma 0.241, 0.467, 5.45, 0.053 Datasheet
NF‐light 103186 Human CSF, Serum, Plasma 0.174 Datasheet
NF‐light 103186 Mouse Serum, Plasma 0.174 Datasheet
NSE 102475 Human CSF, Serum, Plasma 9.88 Datasheet
NT-proBNP 102713 Human Serum, Plasma 0.206 Datasheet
pTau 181 103714 Human CSF, Serum, Plasma 0.085 Datasheet
pTau 231 102292 Human CSF 1.83 Datasheet
PD-1 102292 Human Serum, Plasma 0.879 Datasheet
PD-L1 102648 Human Serum, Plasma 0.105 Datasheet
PIGF 102318 Human Serum, Plasma 0.30 Datasheet
p-NF-heavy 102669 Human CSF, Serum, Plasma 2.88 Datasheet
p-NF-heavy 102669 Mouse Serum, Plasma 2.88 Datasheet
PSA 101478 Human Serum 0.024 Datasheet
Tau 101552 Human CSF, Serum, Plasma 0.062 Datasheet
Tau 10229 Mouse CSF, Serum, Plasma 0.823 Datasheet
TDP-43 103293 Human CSF, Serum, Plasma 8.23 Datasheet
TGF alpha 101863 Human Serum, Plasma 0.207 Datasheet
TGF beta 101984 Human Serum, Plasma 0.514 Datasheet
TNF alpha 101580 Human Serum, Plasma 0.034 Datasheet
TNF alpha 102100 Mouse Serum, Plasma 1.23 Datasheet
TNF beta 102091 Human Serum, Plasma 0.150 Datasheet
TRAIL 100906 Human Serum, Plasma 0.0177 Datasheet
Troponin-I 101588 Human Serum, Plasma 0.079 Datasheet
UCH-L1 102343 Human CSF, Serum, Plasma 3.43 Datasheet
VEGF 102794 Human Serum, Plasma 0.137 Datasheet

 


 

 

 

All assay services are performed in our GLP/GCP-certified laboratory.