Low Sample Volumes

The available sample volume for large-molecule bioanalytical sample testing support could be limited for a variety of reasons.
In preclinical work, animal models such as mouse and rat only allow for relatively small blood volumes to be collected from a single animal.

Rare matrices, for instance cerebrospinal fluid (CSF), synovial fluid or ocular biofluids such as aqueous or vitreous humor, are very limited in volume size - even in the cases of larger animal models or human donors in clinical trials.

Therefore, Microsampling offers a great advantage in preclinical and clinical trials.


Chimera Biotec has specialized in immunoassay sample testing support for studies involving rare matrices and microsampling or with otherwise considerably limited sample volumes.

As experts in ultra sensitive bioanalysis, we have excellent capabilities and vast experience in modifying assays to fulfill the study’s sensitivity requirements while maintaining extremely low sample consumption.



We help you overcome the hurdle of the correlation of required sample volume vs. assay sensitivity

Our AnySource® technology allows parallel testing of various analytes, each at the desired sensitivity and minimal sample consumption of a few µl per duplicate run. Best possible specificity is ensured, as multiplex-typical antibody cross-talk is avoided.

Any Imperacer® assay can be adapted towards minimal sample consumption to support microsampling, microdosing, and dried blood spot (DBS) trials. Conserve your sample, while still maintaining sensitivity beyond the scope of conventional ELISA-type techniques.



Dream Team Combination: Imperacer® and AnySource®

Using our AnySource® Dilution Technology to reduce background, we can make more out of small sample volumes, but keep ultra sensitive detection by exponential signal amplification via Imperacer®.




Rare Matrices

We have gained experience with analytical data on biomarkers from various matrices:

             ♦  Serum/ Plasma
             ♦  CSF (also artificial)
             ♦  Cell culture/ medium
             ♦  Tissue extract
             ♦  Synovial fluid (also artificial)
             ♦  Mouse tail vein
             ♦  Tears
             ♦  Saliva
             ♦  Eye chamber water
             ♦  and many more


Virtually any matrix can be explored utilizing the Imperacer® platform's outstanding ability to maintain extreme sensitivity despite high sample dilution.

Depending on sensitivity requirements, typically 10 analytes (e.g. drug plus 9 biomarkers) can be tested from 25 µl of sample. Taking backup volume into account, 50 µl of neat sample volume is sufficient for testing of 10 analytes in parallel, duplicate well assays with single digit pg/ml sensitivities each.



Diseases and Indications

We have a long track record of supporting trials targeting a wide range of disease areas including:

             ♦  oncology and neurology (CNS)
             ♦  auto-immune
             ♦  inflammatory
             ♦  infectious
             ♦  metabolic
             ♦  vaccines