MSD™ - Meso Scale Discovery

MSD-ECL from Meso Scale Discovery is a proven electrochemiluminescence immunoassay platform for large molecule analysis. The ECL technology enables high sensitivity levels combined with broad assay range, making MSD™ ideal for immunogenicity testing, multiplex biomarker screening and cytokine assays.

We offer MSD™ immunoassays under GLP, GCP and non-regulated.

Chimera Biotec offers full MSD™ testing service for all your bioanalytical needs. We use Ready to use off-the-shelf kits which are available for various biomarkers or develop an highly sensitive MSD™ assay based on your specific requirements.

 


MSD™ Technology

 

In ECL immunoassays, the intensity of light signals emitted due to electricity applied to the plate electrodes is measured. Tripropylamine-containing buffers catalyze a dual redox reaction with Ruthenium emitting light at 620 nm. The MSD-ECL platform uses SULFO-TAG labeled antibodies - and since only labels near the electrode surface are detected - ensures low background noise.

Compared to traditional ELISA methods, the amplified MSD electrochemiluminescence labels yield higher sensitivity, a broad dynamic range and multiplex arrays allow the detection of several analytes from a single sample (e.g. whole blood, serum, plasma, supernatant).Low background and low interference also enable the quantification of analytes in complex matrices.

 

 


 

S-PLEX Technology

Modifying the standard MSD™ procedure, the TURBO-TAG label allows us to provide MSD service of improved sensitivity.

The detection antibody is labeled with TURBO-BOOST which is enhanced in an additional step and then bound by an electrochemiluminescence TURBO-TAG molecule. Additional S-PLEX reagents reduce non-specific signals.

Using the MSD GOLD Read Buffer, the applied voltage causes the captured TURBO-TAG labels to emit light.

 

 


MSD Applications

 

Biomarker

 

Pharmacokinetic

Pharmacodynamic

 

ADA/Immunogenicity

 

 

 

Chimera’s MSD™ expertise

 

Platform intrinsic advantages Our services at a glance
  • Highly sensitive assays
  • Fast and automated platform
  • Wide range of assay kits
  • In-house antibody pair testing
  • Assay development services
  • Multiplex biomarker assays
  • PK assays
  • Assay transfer
  • Assay validation (fit-for-purpose, non-reg., GLP confirm validation)
  • Regulated bioanalysis (non-reg / GCP / GLP)

Take advantage of Chimera Biotec‘s more than 20 years of in-depth experience as a specialty CRO for immunoassays which go beyond the scope of what is usually considered feasible.

MSD S-Plex (MSD):

S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. Thes low detection limits enable the measurement of analytes at lower concentrations, reduce sample volume required, and reduce the amount of critical reagents used. The S-PLEX assay format builds on MSD consumables and instruments and leverages MSD’s assay development expertise by using a procedure that is similar to other MSD assay methods.

Benefit from the best in class in immunoassay sensitivity!

 


 

MSD™ immunoassay services:

Our kit-based biomarker portfolio for MSD™. If you can not find your biomarker of interest, we develop a highly specific custom assay for you. Just contact our team of experts.

Target Catalog No. Species Matrix LLOD (pg/ml) LLOQ - ULOQ (pg/ml) Datasheet
A-beta 38 (4G8) K150SHE Human Cell Culture Supernatant, Tissue Homogenate, CSF 22.2 60 - 7,500 Datasheet
A-beta 38 (4G8) K150SHE Rat Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, CSF 22.2 60 - 7,500 Datasheet
A-beta 38 (4G8) K150SHE Mouse Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, CSF 22.2 60 - 7,500 Datasheet
A-beta 38 (6E10) K150SIE Human Cell Culture Supernatant, Tissue Homogenate, CSF 16.7 60 - 8,480 Datasheet
A-beta 40 (4G8) K150SJE Human Cell Culture Supernatant, Tissue Homogenate, CSF 5.41 20 - 6,000 Datasheet
A-beta 40 (4G8) K150SJE Rat Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, CSF 5.41 20 - 6,000 Datasheet
A-beta 40 (4G8) K150SJE Mouse Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, CSF 5.41 20 - 6,000 Datasheet
A-beta 40 (6E10) K150SKE Human Cell Culture Supernatant, CSF, Tissue Homogenate 9.97 50 - 7,000 Datasheet
A-beta 42 (4G8) K150SLE Human Cell Culture Supernatant, Tissue Homogenate, CSF 0.516 2.5 - 1,270 Datasheet
A-beta 42 (4G8) K150SLE Rat Cell Culture Supernatant, Serum, Plasma, CSF, Tissue Homogenate 0.516 2.5 - 1,270 Datasheet
A-beta 42 (4G8) K150SLE Mouse Cell Culture Supernatant, Serum, Plasma, CSF, Tissue Homogenate 0.516 2.5 - 1,270 Datasheet
A-beta 42 (6E10) K151LBE Human CSF 0.368 3.13 - 1,270 Datasheet
CRP K151STD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.33 27.6 - 49,600 Datasheet
Eotaxin K151NSD Human Serum, Plasma, Urine, Cell Culture Supernatant 3.26 12.3 - 1,120 Datasheet
Eotaxin-3 K151NUD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.77 10.2 - 3,750 Datasheet
Eotaxin-3 K156NUD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 1.77 10.2 - 3,750 Datasheet
FGF (basic) K151MDD Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 0.09 2.6 - 1,780 Datasheet
Flt-1/VEGFR-1 K151RZD Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 0.90 10 - 6,410 Datasheet
GLP-1 Active*** K1503OD Human Cell Culture Supernatant, EDTA Plasma, Plasma 0.02 0.30 - 120 Datasheet
GLP-1 Active*** K1503OD Non-human Primate (NHP) Cell Culture Supernatant, EDTA Plasma, Plasma 0.02 0.30 - 120 Datasheet
GLP-1 Active*** K1503OD Mouse Cell Culture Supernatant, EDTA Plasma, Plasma 0.02 0.30 - 120 Datasheet
GLP-1 Active*** K1503OD Rat Cell Culture Supernatant, EDTA Plasma, Plasma 0.02 0.30 - 120 Datasheet
GLP-1 Active*** K1503OD Canine Cell Culture Supernatant, EDTA Plasma, Plasma 0.02 0.30 - 120 Datasheet
GLP-1 Total*** K1503PD Human Plasma, Cell Culture Supernatant 0.017 0.18 - 120 Datasheet
GLP-1 Total*** K1503PD Non-human Primate (NHP) Plasma, Cell Culture Supernatant 0.017 0.18 - 120 Datasheet
GLP-1 Total*** K1503PD Mouse Plasma, Cell Culture Supernatant 0.017 0.18 - 120 Datasheet
GLP-1 Total*** K1503PD Rat Plasma, Cell Culture Supernatant 0.017 0.18 - 120 Datasheet
GLP-1 Total*** K1503PD Canine Plasma, Cell Culture Supernatant 0.017 0.18 - 120 Datasheet
GM-CSF K151RID Human Serum, Plasma, Urine, Cell Culture Supernatant 0.16 0.842 - 750 Datasheet
GM-CSF K156RID Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.16 0.842 - 750 Datasheet
ICAM-1 K151SUD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.94 15.0 - 32,700 Datasheet
IFN-gamma K151QOD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.37 1.76 - 938 Datasheet
IFN-gamma K156QOD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.37 1.76 - 938 Datasheet
IFN-gamma K153QOD Rat Serum, Plasma, Urine, Cell Culture Supernatant 0.65 39.7 - 3,750 Datasheet
IFN-gamma K152QOD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.04 0.39 - 570 Datasheet
IL-1 alpha K151RBD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.09 2.85 - 278 Datasheet
IL-1 beta K151QPD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.05 0.646 - 375 Datasheet
IL-1 beta K156QPD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.05 0.646 - 375 Datasheet
IL-1 beta K153QPD Rat Serum, Plasma, Urine, Cell Culture Supernatant 6.92 102 - 8,100 Datasheet
IL-1 beta K152QPD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.11 0.72 - 1,030 Datasheet
IL-10 K151QUD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.04 0.298 - 233 Datasheet
IL-10 K156QUD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.04 0.298 - 233 Datasheet
IL-10 K153QUD Rat Serum, Plasma, Urine, Cell Culture Supernatant 16.4 163 - 15,670 Datasheet
IL-10 K152QUD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.94 7.26 - 2,030 Datasheet
IL-12/IL-23p40 K151RJD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.33 1.32 - 2,250 Datasheet
IL-12/IL-23p40 K156RJD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.33 1.32 - 2,250 Datasheet
IL-12p70 K151QVD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.11 1.22 - 315 Datasheet
IL-12p70 K152QVD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 9.95 179 - 20,600 Datasheet
IL-13 K151ODD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.24 4.21 - 353 Datasheet
IL-13 K153ODD Rat Serum, Plasma, Urine, Cell Culture Supernatant 1.97 12.5 - 1,080 Datasheet
IL-15 K151RDD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.15 0.774 - 525 Datasheet
IL-15 K156RDD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.15 0.774 - 525 Datasheet
IL-15 K152RDD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 16 43.2 - 26,000 Datasheet
IL-16 K151RED Human Serum, Plasma, Urine, Cell Culture Supernatant 2.83 19.1 - 1,870 Datasheet
IL-16 K156RED Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 2.83 19.1 - 1,870 Datasheet
IL-17A K151RFD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.31 3.19 - 3,650 Datasheet
IL-17A K156RFD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.31 3.19 - 3,650 Datasheet
IL-17A (Gen. B) K151WMD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.413 5.86 - 1,950 Datasheet
IL-17A/F K151WND Human Serum, Plasma, Urine, Cell Culture Supernatant 0.930 7.57 - 3,900 Datasheet
IL-17A/F K152WND Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.23 1.39 - 1,620 Datasheet
IL-17B K151WOD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.185 1.12 - 1,040 Datasheet
IL-17C K151WPD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.682 3.84 - 1,620 Datasheet
IL-17D K151WQD Human Serum, Plasma, Urine, Cell Culture Supernatant 3.87 11.2 - 5,200 Datasheet
IL-1RA K151WTD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.12 9.19 - 650 Datasheet
IL-2 K151QQD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.09 0.890 - 938 Datasheet
IL-2 K156QQD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.09 0.890 - 938 Datasheet
IL-2 K152QQD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.22 1.03 - 1,570 Datasheet
IL-21 K151WUD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.193 1.65 - 650 Datasheet
IL-22 K151WVD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.27 2.78 - 325 Datasheet
IL-23 K151WWD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.274 4.60 - 3,250 Datasheet
IL-27 K151WXD Human Serum, Plasma, Urine, Cell Culture Supernatant 4.2 38.7 - 13,000 Datasheet
IL-27p28/IL-30 K152WXD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 1.39 5.91 - 6,500 Datasheet
IL-3 K151WZD Human Serum, Plasma, Urine, Cell Culture Supernatant 2.37 12.6 - 1,950 Datasheet
IL-31 K151XAD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.446 4.22 - 650 Datasheet
IL-33 K152XBD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.36 1.85 - 1,950 Datasheet
IL-4 K151QRD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.02 0.218 - 158 Datasheet
IL-4 K153QRD Rat Serum, Plasma, Urine, Cell Culture Supernatant 0.69 8.0 - 723 Datasheet
IL-4 K152QRD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.11 0.818 - 1,060 Datasheet
IL-5 K151QSD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.14 4.41 - 562 Datasheet
IL-5 K156QSD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.14 4.41 - 562 Datasheet
IL-5 K153QSD Rat Serum, Plasma, Urine, Cell Culture Supernatant 14.1 82 - 3,000 Datasheet
IL-5 K152QSD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.06 0.302 - 590 Datasheet
IL-6 K151QXD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.06 0.633 - 488 Datasheet
IL-6 K156QXD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.06 0.633 - 488 Datasheet
IL-6 K153QXD Rat Serum, Plasma, Urine, Cell Culture Supernatant 13.8 96.9 - 8,550 Datasheet
IL-6 K152QXD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.61 7.61 - 3,140 Datasheet
IL-7 K151RCD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.12 0.546 - 563 Datasheet
IL-7 K156RCD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.12 0.546 - 563 Datasheet
IL-8 K151RAD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.07 0.591 - 375 Datasheet
IL-8 K156RAD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.07 0.591 - 375 Datasheet
IL-9 K151XCD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.311 2.23 - 975 Datasheet
IL-9 K152XCD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 3.84 21.9 - 2,600 Datasheet
IP-10 K151NVD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.37 1.37 - 500 Datasheet
IP-10 K156NVD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.37 1.37 - 500 Datasheet
IP-10 K152QUD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.32 2.15 - 650 Datasheet
KC/GRO K153QTD Rat Serum, Plasma, Urine, Cell Culture Supernatant 1.04 21.7 - 728 Datasheet
KC/GRO K152QTD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.24 3.29 - 1,230 Datasheet
MCP-1 K151NND Human Serum, Plasma, Urine, Cell Culture Supernatant 0.090 1.09 - 375 Datasheet
MCP-1 K156NND Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.09 1.09 - 375 Datasheet
MCP-1 K152NND Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.672 4.42 - 325 Datasheet
MCP-4 K151NOD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.69 5.13 - 469 Datasheet
MCP-4 K156NOD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 1.69 5.13 - 469 Datasheet
MDC K151NPD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.22 88.3 - 7,500 Datasheet
MDC K156NPD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 1.22 88.3 - 7,500 Datasheet
MIP-1 alpha K151NQD Human Serum, Plasma, Urine, Cell Culture Supernatant 3.02 13.8 - 743 Datasheet
MIP-1 alpha K156NQD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 3.02 13.8 - 743 Datasheet
MIP-1 alpha K152NQD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.081 0.380 - 390 Datasheet
MIP-1 beta K151NRD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.17 1.02 - 750 Datasheet
MIP-1 beta K156NRD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.17 1.02 - 750 Datasheet
MIP-2 K152QCD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.053 0.580 - 423 Datasheet
MIP-3 alpha K151XDD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.05 0.588 - 325 Datasheet
PlGF K151MED Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 0.21 1.5 - 800 Datasheet
SAA K151SSD Human Serum, Plasma, Urine, Cell Culture Supernatant 10.9 54.1 - 138,000 Datasheet
TARC K151NTD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.22 3.32 - 1,120 Datasheet
TARC K156NTD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.22 3.32 - 1,120 Datasheet
Tau K151LAE Human CSF 10.18 30 - 8,000 Datasheet
Tie-2 K151KWD Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 31.3 396 - 63,400 Datasheet
TNF-alpha K151QWD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.04 0.690 - 248 Datasheet
TNF-alpha K153QWD Rat Serum, Plasma, Urine, Cell Culture Supernatant 0.72 9.1 - 793 Datasheet
TNF-alpha K152QWD Mouse Serum, Plasma, Urine, Cell Culture Supernatant 0.13 0.97 - 403 Datasheet
TNF-beta K151RGD Human Serum, Plasma, Urine, Cell Culture Supernatant 0.08 0.465 - 458 Datasheet
TNF-beta K156RGD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 0.08 0.465 - 458 Datasheet
TSLP K151XED Human Serum, Plasma, Urine, Cell Culture Supernatant 0.063 0.460 - 325 Datasheet
VCAM-1 K151SRD Human Serum, Plasma, Urine, Cell Culture Supernatant 6.00 37.6 - 32,000 Datasheet
VEGF-A K151RHD Human Serum, Plasma, Urine, Cell Culture Supernatant 1.12 7.70 - 562 Datasheet
VEGF-A K156RHD Non-human Primate (NHP) Serum, Plasma, Urine, Cell Culture Supernatant 1.12 7.70 - 562 Datasheet
VEGF-C K151LTD Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 9.91 146 - 17,500 Datasheet
VEGF-D K151LUD Human Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate 4.36 67.1 - 18,800 Datasheet

 


 

 

All assay services are performed in our GLP/GCP-certified laboratory.