Immuno-PCR

Imperacer®, the award-winning Immuno-PCR (IPCR) based ligand-binding assay platform. Chimera’s proprietary ultra sensitive immunoassay technology that combines the exquisite of ELISA-based antibody specificity with the most sensitive detection system in molecular biology: real-time qPCR.
The exponential signal amplification dramatically improves sensitivity and enables sub pg/ml detection.

Imperacer® assays combine ultra sensitivity with excellent precision, higher than usual drug tolerance, and minimal matrix effects. It´s broad assay range typically reduces the number of required sample dilutions to one, with no hook effect within physiological relevant concentrations.

We offer Imperacer® immunoassays under GLP, GCP and non-regulated.

Chimera Biotec offers full Immuno-PCR testing service for all your bioanalytical needs. Check out our Biomarker Menu for available validated assays. We also develop an ultra-sensitive Imperacer® assay based on your specific requirements.


 

Imperacer® Technology

 

 

In our Immuno-PCR technique, patented antibody-DNA-conjugates are used as detection reagents for the captured analyte of interest.

The following real-time PCR readout allows extremely powerful signal amplification of the DNA-marker and leads to a up to 1000-fold increase in sensitivity, as compared to any standard ELISA.

This ultra sensitivity enables sub pg/ml detection in challenging matrices (blood, salvia, CSF, intraocular fluid and more) with only small assay volume consumption.

Read our case studies and publications to learn more about Imperacer®.

 

 

Our proprietary immunoassay platform Imperacer® combines the preeminent specificity of antibody-antigen binding with the extremely powerful signal amplification of DNA analytics, using our patented antibody-DNA conjugates.
A double-stranded DNA-tag allows for exponential signal amplification of immunoassay binding events by PCR. This exponential amplification results in ultra sensitive assays with very broad dynamic ranges and - in consequence - to very dilution tolerant assays.

Imperacer® allows modification of the most sensitive assay procedure toward an assay with the minimal sample volume requirement at the needed level of sensitivity by customized AnySource® sample dilution.
Sensitivity can be employed for immunoassay quantification from microliter (µl) volumes of neat sample, still maintaining the needed sensitivity requirement of the study in trials involving microsampling or rare matrix samples.

 

 

Ultra sensitive in 5 steps

Step 1: 96-well plates are coated with capture antibody

Step 2: Blocking solution reduces unspecific background

Step 3: Adding samples induces analyte binding

Step 4: Chimera´s proprietary DNA-antibody detection
             conjugates form the other half of the complex

Step 5: Real time qPCR exponentially amplifies signal intensity

Chimera´s proprietary AnySource® technology allows high sample dilution to reduce background to a minimum - but still detecting ultra sensitively with our DNA-antibody conjugates.

 


 

Immuno-PCR Applications

Imperacer®´s broad assay range of 3-6 log units allows us to perform all your studies – from TOX to clinic - on one platform.

 

Biomarker

 

Pharmacokinetic

Pharmacodynamic

 

ADA/Immunogenicity

 

 

Chimera's Immuno-PCR expertise

We support your study design with ultra sensitive sample testing, courtesy of exponential, PCR driven signal amplification. Developed by the pioneers of the Immuno-PCR, Chimera Biotec’s proprietary Imperacer® technology brings this concept to professional bioanalytical sample testing.

 

Platform intrinsic advantages Our services at a glance
  • Exponential signal amplification
  • Ultra sensitive platform
  • Extremely broad assay range
  • Microsampling approach
  • In-house antibody pair testing
  • Assay development services
  • PK assays
  • Biomarker assays
  • Assay transfer
  • Assay validation (fit-for-purpose, non-reg., GLP confirm validation)
  • Regulated bioanalysis (non-reg / GCP / GLP)

 

Take advantage of Chimera Biotec‘s more than 20 years of in-depth experience as a specialty CRO for immunoassays which go beyond the scope of what is usually considered feasible.

Benefit from the best in class in immunoassay sensitivity!

 


 

Imperacer® Assays

Qualified
Qualified assays were developed, optimized and validated for specific reagents and matrices.

Offered
Offered assays can be activated on request.
The assay setup is on us, your commitment starts with the validation, which we perform resective to your purpose and under the quality regime of your choice - from internal decision making to full industry support according to guidances. A small assay activation fee will be used as a credit for the following validation and bioanalysis.

 

Target

Species

Matrix

LLOQ

pg/ml

ULOQ

pg/ml

Status

alpha Synuclein Human Serum, Plasma     offered
Amphiregulin Human, Mouse Serum, Plasma     offered
Angiopoietin 1 (ANG-1) Human, Mouse Serum, Plasma     offered
Angiopoietin 2 (ANG-2)

Human

Human

Aqueous humor

Serum, Plasma

6.00 5,000.00

qualified

offered

Angiopoietin-like 4 Human Serum, Plasma     offered
APP Human Serum, Plasma     offered
BCMA Human Serum, Plasma     offered
BDNF Human, Mouse Serum, Plasma     offered
Cathepsin L Human Serum, Plasma     offered
Cathepsin S Human Serum, Plasma     offered

CCL4 (MIP-1)

Human Serum, Plasma     offered
CCL11 (Eotaxin) Mouse Serum, Plasma     offered
CCL17 (TARC) Human Serum, Plasma     offered
CCL18 (PARC) Human Serum, Plasma     offered
CCL19 (MIP-3 beta) Human Serum, Plasma     offered
CCL22 (MDC) Human Serum, Plasma     offered
CD14 Human, Mouse Serum, Plasma     offered
CD40 ligand Human, Mouse Serum, Plasma     offered
C-Met Human, Mouse Serum, Plasma     offered
CRP

Human

Human, Mouse

Serum

Serum, Plasma

2,700.00 1,046,000.00

qualified

offered

CX3CL2 (Fractalkine) Human Serum, Plasma     offered
CXCL1 (KC) Mouse Serum, Plasma     offered
CXCL9 (MIG) Human Serum, Plasma     offered
CXCL12 (SDF-1) Human, Mouse Serum, Plasma     offered
CXCL 13

Human

Human, Mouse

CSF

Serum, Plasma

0.79 300.00

qualified

offered

E-Cadherin Human, Mouse Serum, Plasma     offered
EGF Human, Mouse Serum, Plasma     offered
EGFR Human, Mouse Serum, Plasma     offered
Eotaxin (CCL11) Human, Mouse Serum, Plasma     offered
ErbB3 (Her3) Human Serum, Plasma     offered
FGF-19 Human Serum, Plasma     offered
FGF-2 Human Serum, Plasma     offered
FGF basic Human, Mouse Serum, Plasma     offered
G-CSF Human, Mouse Serum, Plasma     offered
GDF-15 Human Serum, Plasma     offered
GFAP Human Serum, Plasma     offered
GM-CSF

Human

Human, Mouse

Plasma

Serum, Plasma

0.06 36.70

qualified

offered

gp130 Human, Mouse Serum, Plasma     offered
HGF Human Serum, Plasma     offered
HIF-1 alpha total Human, Mouse Serum, Plasma     offered
HSP70 Human, Mouse Serum, Plasma     offered
ICAM-1 (CD54) Human, Mouse Serum, Plasma     offered
IFN beta Human, Mouse Serum, Plasma     offered
IFN gamma

Human

Human, Mouse, Rat

Plasma

Serum, Plasma

0.03 153.36

qualified

offered

IGF-1 Human Serum, Plasma     offered
IL-1 alpha

Human

Human, Mouse

Synovial fluid

Serum, Plasma

0.30 1,000.00

qualified

offered

IL-1 beta

Human

Human, Mouse, Rat

Synovial fluid

Serum, Plasma

0.12 75.00

qualified

offered

IL-1ra (IL-1F3) Human Serum, Plasma     offered
IL-2

Human

Human, Mouse

Serum

Serum, Plasma

0.46 1,000.00

qualified

offered

IL-2 R alpha Human, Mouse Serum, Plasma     offered
IL-6 Human Serum 0.31 5,000.00 qualified
IL-6R Human Plasma 610.00 150,000.00 qualified
IL-10

Human

Human, Mouse, Rat

Plasma

Serum, Plasma

9.00 19,683.00

qualified

offered

IL-11 Human, Mouse Serum, Plasma     offered
IL-12 p70 Human, Mouse Serum, Plasma     offered
IL-13 Human, Mouse Serum, Plasma     offered
IL-15 Human, Mouse Serum, Plasma     offered
IL-16 Human, Mouse Serum, Plasma     offered
IL-17 Human, Mouse Serum, Plasma     offered
IL-18 Monkey Serum, Plasma     offered
IL-18 total Human Serum, Plasma     offered
IL-19 Human Serum, Plasma     offered
IL-21 Mouse Serum, Plasma     offered
IL-22 Human, Mouse Serum, Plasma     offered
IL-23 Human, Mouse Serum, Plasma     offered
IL-25

Human

Human, Mouse

Serum

Serum, Plasma

3.00 2,190.00

qualified

offered

IL-27 Human, Mouse Serum, Plasma     offered
IL-28A Human, Mouse Serum, Plasma     offered
IL-3 Human, Mouse Serum, Plasma     offered
IL-33 Human, Mouse Serum, Plasma     offered
IL-36 beta Human Serum, Plasma     offered
IL-4 Human, Mouse Serum, Plasma     offered
IL-5 Human, Mouse Serum, Plasma     offered
IL-6 Human, Mouse, Rat Serum, Plasma     offered
IL-6R Human, Mouse Serum, Plasma     offered
IL-7 Human, Mouse Serum, Plasma     offered
IL-8 (CXCL8) Human, Mouse Serum, Plasma     offered
Leptin Human, Mouse Serum, Plasma     offered
LIF Human, Mouse Serum, Plasma     offered
Lipocalin-2 (NGAL) Human Serum, Plasma     offered
MCP (CCL2) Human, Mouse Serum, Plasma     offered
MIF Human Serum, Plasma     offered
MIP-3 alpha (CCL20) Human, Mouse Serum, Plasma     offered
Osteopontin (OPN) Human Serum, Plasma     offered
PDGF-BB Human Serum, Plasma     offered
pEGFR Human Serum, Plasma     offered
Periostin (OSF-2) Human Serum, Plasma     offered
Prolactin Human, Mouse Serum, Plasma     offered
PSA (KLK-3) Human Serum, Plasma     offered
pTau Human Plasma 4.00 8,748.00 qualified
Serpin F1 (PEDF) Human Serum, Plasma     offered
TGF alpha Human Serum, Plasma     offered
TGF beta 1 Human, Mouse Serum, Plasma     offered
TGF beta 3 Human Serum, Plasma     offered
TIMP-1 Human Serum, Plasma     offered
TNF RI Human, Mouse Serum, Plasma     offered
TNF alpha

Human

Human, Mouse, Rat

Plasma

Serum, Plasma

1.17 150.00

qualified

offered

Troponin-I Human Serum 9.14 20,000.00 qualified
UCH-L1 Human Serum, Plasma     offered
VCAM (CD106) Human Serum, Plasma     offered
VE-Cadherin Human Serum, Plasma     offered
VEGF

Human

Human, Mouse

Aqueous humor

Serum, Plasma

2.50 10,000.00

qualified

offered

VEGF R1 Human Serum, Plasma     offered

 


 

 

All assay services are performed in our GLP/GCP-certified laboratory.