High sensitive ELISA

ELISA (Enzyme linked immunosorbent assays) is the gold standard for the quantification of proteins in ligand binding assays. The use of Chimera’s proprietary multimeric conjugates together with modern substrates opens the door for highly sensitive target quantification.

We offer ELISA immunoassays under GLP, GCP and non-regulated.

Chimera Biotec offers full ELISA testing service for all your bioanalytical needs. We use Ready to use off-the-shelf kits which are available for various biomarkers or develop an highly sensitive ELISA based on your specific requirements.

 


 

ELISA Technology

 

Sample antigens attached to the plate´s surface are incubated with a target-specific antibody which are linked to an enzyme. Adding the enzyme´s substrate results in a detectable signal, most likely a change in color.

The ELISA platform is available in differented types - Direct ELISA, Sandwich ELISA and Competetive ELISA - each with its own advantages for the experimental set up. Our team of experts will pick the ideal mechanism for your study requirements to ensure optimal sample testing.

 


 

ELISA Applications

 

Biomarker

 

Pharmacokinetic

Pharmacodynamic

 

ADA/Immunogenicity

 


 

 

Chimera’s ELISA expertise

 

Platform intrinsic advantages Our services at a glance
  • Highly sensitive assays
  • Fast and automated platform
  • Wide range of assay kits
  • In-house antibody pair testing
  • Assay development services
  • Biomarker assays
  • PK assays
  • Assay transfer
  • Assay validation (fit-for-purpose, non-reg., GLP confirm validation)
  • Regulated bioanalysis (non-reg / GCP / GLP)

 

Take advantage of Chimera Biotec‘s more than 20 years of in-depth experience as a specialty CRO for immunoassays which go beyond the scope of what is usually considered feasible.

High sensitivity ELISA (HS ELISA):

Our expertise in detection conjugate synthesis and state of the art signal amplification chemistry allows us to optimize ELISA performance. Starting from commercially available kits and reagents, we provide cost effective biomarker quantification to fulfill your requirements within ELISA technical limitations. Custom PK assay development based on your drug specific reagents or uncommon biomarkers is available up to GLP/GCP conform validation and bioanalysis.

Benefit from the best in class in immunoassay sensitivity!

 

Target

Species

Matrix

Status

alpha Synuclein Human Serum, Plasma offered
Amphiregulin Human, Mouse Serum, Plasma offered
Angiopoietin 1 (ANG-1) Human, Mouse Serum, Plasma offered
Angiopoietin 2 (ANG-2) Human Serum, Plasma offered
Angiopoietin-like 4 Human Serum, Plasma offered
APP Human Serum, Plasma offered
BCMA Human Serum, Plasma offered
BDNF Human, Mouse Serum, Plasma offered
Cathepsin L Human Serum, Plasma offered
Cathepsin S Human Serum, Plasma offered
CCL2 (MCP-1) Human, Mouse Serum, Plasma offered
CCL4 (MIP-1) Human Serum, Plasma offered
CCL11 (Eotaxin) Mouse Serum, Plasma offered
CCL17 (TARC) Human Serum, Plasma offered
CCL18 (PARC) Human Serum, Plasma offered
CCL19 (MIP-3 beta) Human Serum, Plasma offered
CCL20 (MIP-3 alpha) Human, Mouse Serum, Plasma offered
CCL22 (MDC) Human Serum, Plasma offered
CD14 Human, Mouse Serum, Plasma offered
CD25 (IL-2 R alpha) Human, Mouse Serum, Plasma offered
CD40 ligand Human, Mouse Serum, Plasma offered
CD54 (ICAM-1) Human, Mouse Serum, Plasma offered
CD106 (VCAM-1) Human Serum, Plasma offered
C-Met Human, Mouse Serum, Plasma offered
CRP Human, Mouse Serum, Plasma offered
CX3CL2 (Fractalkine) Human Serum, Plasma offered
CXCL1 (KC) Mouse Serum, Plasma offered
CXCL9 (MIG) Human Serum, Plasma offered
CXCL12 (SDF-1) Human, Mouse Serum, Plasma offered
CXCL13 Human, Mouse Serum, Plasma offered
E-Cadherin Human, Mouse Serum, Plasma offered
EGF Human, Mouse Serum, Plasma offered
EGFR Human, Mouse Serum, Plasma offered
Eotaxin (CCL11) Human, Mouse Serum, Plasma offered
ErbB3 (Her3) Human Serum, Plasma offered
FGF-19 Human Serum, Plasma offered
FGF-2 Human Serum, Plasma offered
FGF basic Human, Mouse Serum, Plasma offered
G-CSF Human, Mouse Serum, Plasma offered
GDF-15 Human Serum, Plasma offered
GFAP Human Serum, Plasma offered
GM-CSF Human, Mouse Serum, Plasma offered
gp130 Human, Mouse Serum, Plasma offered
HGF Human Serum, Plasma offered
HIF-1 alpha total Human, Mouse Serum, Plasma offered
HSP70 Human, Mouse Serum, Plasma offered
ICAM-1 (CD54) Human, Mouse Serum, Plasma offered
IFN beta Human, Mouse Serum, Plasma offered
IFN gamma Human, Mouse, Rat Serum, Plasma offered
IGF-1 Human Serum, Plasma offered
IL-1 alpha Human, Mouse Serum, Plasma offered
IL-1 beta Human, Mouse, Rat Serum, Plasma offered
IL-1ra (IL-1F3) Human Serum, Plasma offered
IL-2 Human, Mouse Serum, Plasma offered
IL-2 R alpha Human, Mouse Serum, Plasma offered
IL-10 Human, Mouse, Rat Serum, Plasma offered
IL-11 Human, Mouse Serum, Plasma offered
IL-12 p70 Human, Mouse Serum, Plasma offered
IL-13 Human, Mouse Serum, Plasma offered
IL-15 Human, Mouse Serum, Plasma offered
IL-16 Human, Mouse Serum, Plasma offered
IL-17 Human, Mouse Serum, Plasma offered
IL-18 Monkey Serum, Plasma offered
IL-18 total Human Serum, Plasma offered
IL-19 Human Serum, Plasma offered
IL-21 Mouse Serum, Plasma offered
IL-22 Human, Mouse Serum, Plasma offered
IL-23 Human, Mouse Serum, Plasma offered
IL-25 Human, Mouse Serum, Plasma offered
IL-27 Human, Mouse Serum, Plasma offered
IL-28A Human, Mouse Serum, Plasma offered
IL-3 Human, Mouse Serum, Plasma offered
IL-33 Human, Mouse Serum, Plasma offered
IL-36 beta Human Serum, Plasma offered
IL-4 Human, Mouse Serum, Plasma offered
IL-5 Human, Mouse Serum, Plasma offered
IL-6 Human, Mouse, Rat Serum, Plasma offered
IL-6R Human, Mouse Serum, Plasma offered
IL-7 Human, Mouse Serum, Plasma offered
IL-8 (CXCL8) Human, Mouse Serum, Plasma offered
Leptin Human, Mouse Serum, Plasma offered
LIF Human, Mouse Serum, Plasma offered
Lipocalin-2 (NGAL) Human Serum, Plasma offered
MCP (CCL2) Human, Mouse Serum, Plasma offered
MIF Human Serum, Plasma offered
MIP-3 alpha (CCL20) Human, Mouse Serum, Plasma offered
Osteopontin (OPN) Human Serum, Plasma offered
PDGF-BB Human Serum, Plasma offered
pEGFR Human Serum, Plasma offered
Periostin (OSF-2) Human Serum, Plasma offered
Prolactin Human, Mouse Serum, Plasma offered
PSA (KLK-3) Human Serum, Plasma offered
Serpin F1 (PEDF) Human Serum, Plasma offered
TGF alpha Human Serum, Plasma offered
TGF beta 1 Human, Mouse Serum, Plasma offered
TGF beta 3 Human Serum, Plasma offered
TIMP-1 Human Serum, Plasma offered
TNF RI Human, Mouse Serum, Plasma offered
TNF alpha Human, Mouse, Rat Serum, Plasma offered
UCH-L1 Human Serum, Plasma offered
VCAM (CD106) Human Serum, Plasma offered
VE-Cadherin Human Serum, Plasma offered
VEGF Human, Mouse Serum, Plasma offered
VEGF R1 Human Serum, Plasma offered

 

 

 

All assay services are performed in our GLP/GCP-certified laboratory.