Ultra Sensitive Ligand-Binding Assay Capabilities

Bioanalytical assay development for sample testing services beyond the limitations of classical immunoassays.

There is no need to invest in costly laboratory equipment and acquiring internal expertise regarding ultra sensitive platforms!
Continue working on your preferred in-house platform and only outsource case studies where improved sensitivities or related performance criteria is a must.

Chimera Biotec is equipped and accustomed to developing ultra sensitive assays from your specifications to fulfill your requirements. As pioneers for ultra sensitive immunoassays and with the longest track record in the CRO industry for ultra sensitive bioanalytics, we specialize in case studies where standard ELISA quantification is just not powerful enough.


 

 

With our expert knowledge in sample dilution – based on our proprietary AnySource® technology – we can utilize sensitivity to tailor your assay towards alternate needs, such as minimal sample consumption for bioanalytical studies involving microsampling or rare matrices. 

 


 

Excellence in immunoassay development, validation and bioanalysis is our core motivation on all our platforms.

 

Especially in the ultra sensitive realm, construction, validation and reproducible production of standards (STD) and corresponding quality control samples (QCs) for accurate quantification is key to fulfilling study needs and regulatory requirements.

Biomarkers and biotherapeutic drugs, however, have the potential to interact with the STD-curve matrix in which the analyte is spiked.

 

To avoid dilution effects when spiking the analyte
from stock storage buffer into the STD-curve matrix
and to avoid serial dilution effects, Chimera works with
dispenser technology down to the femto-liter (fl) volume range.

This allows direct spiking of a particular STD or QC concentration
into STD-curve matrix without the introduction of serial dilution effects.

 

 

How biological specimens are handled is also of great importance - particularly in rare matrix or microsampling study support – wherever limitations on sample volume availability apply.

Here at Chimera Biotec we take your sample very seriously. Since even state of the art laboratory equipment can only be as good as the person operating it, our experienced laboratory staff receive ongoing training.
They also individually calibrate their own pipettes to ensure accurate pipetting of sample volumes as low as 0.5 µl (500 nl). Experienced and properly trained personnel, together with constant quality monitoring and improvement is vital for ultra sensitive bioanalytical GLP/GCP services.

 


 

Chimera Biotec: Ultra sensitive bioanalysis & beyond

Large-molecule sample testing support from the industry’s longest standing experts for ultra sensitive bioanalysis

 

Why ultra sensitivity:

While the sensitivities of classic, colorimetric ELISA (enzyme linked immunosorbent assay) are sufficient for bioanalysis in many cases, there is a growing interest in immunoassay techniques whose detection capabilities go beyond those of ELISA. The complexity and novel mechanisms of action of contemporary, biotherapeutic drugs (biologics) increasingly demand ultra sensitive detection methods for both PK sample testing as well as for endogenous or drug-related, low abundance biomarkers.

 

Who we are:

Your GLP/GCP lab in Europe. As pioneers in the field, Chimera Biotec has been specialized in supporting drug development programs that demand the highest bioanalytical quality since 2000. Our long standing expertise in beyond ELISA sensitivity immunoassays enables us to make the most out of any immunoassay platform and provide our customers with accurate and comprehensive scientific results.

 

What we do:

For more than 20 years, Chimera Biotec has specialized in supporting drug development programs with highest demands on bioanalytical quality. We develop and validate ultra sensitive immunoassays in accordance with the regulatory requirements and bioanalytical method validation guidance documents.

Based on your antibody experience on your preferred in-house immunoassay platform, we can run a technology evaluation and feasibility study across our ultra sensitive platforms. The resulting data will allow you to select the platform best-suited to support your study's bioanalytical needs.

 

What makes us different:

With our expert knowledge in sample dilution – based on our proprietary AnySource® technology – we can utilize sensitivity to tailor your assay towards alternate needs, such as minimal sample consumption for bioanalytical studies involving microsampling or rare matrices. 

 

Our quality standards:

Our GLP/GCP certified laboratories deliver bioanalytical services ranging from non-regulated research processing of a few samples to the handling of thousands of samples for clinical phase-III research on time.